Comparison of Bacterial Adhesion on Composite Surfaces, 3D Printed vs Milled vs Conventional: An in vitro study

Description :

The study involves the evaluation of bacterial adhesion on the surfaces of different composites manufactured with the following techniques: conventional direct technique, milling and 3D printing. The different materials will be standardized with the same dimensions and in the same form of a disk. The disks will be divided into 6 groups as follows: Group 1: Conventional without thermocycling Group 2: Milled without thermocycling Group 3: 3D Printed without thermocycling Group 4: Conventional after thermocycling Group 5: Milled after thermocycling Group 6: 3D Printed after thermocycling All specimens will be cleaned with 70% ethanol for 10 seconds and then thoroughly rinsed with sterile distilled water for 10 seconds. The microbiology part will take place. Streptococcus and Staphylococcus bacteria will be used to create a biofilm which will be later applied on the disks. Colony forming units will then be counted and analyzed on each group and will be divided in 3 parts: - Biofilm (streptococcus + staphylococcus) - Streptococcus - Staphylococcus The material with the least bacterial count will be considered the most resistant to bacterial adhesion.