Comparative Evaluation of a Customizable Bone Grafting Putty and DBBM on Osteoblast Differentiation from Umbilical Cord and Dental Pulp Mesenchymal Stem Cells: An In-Vitro Study and a Rats’ Calvaria Model

Description :

This pre-clinical project aims at evaluating the efficacy of a new, customizable bone grafting putty in terms of osteoblastic cells proliferation and bone regeneration. The putty is obtained by mixing a powder (CaCO3, CaCO4, Silicate, β-TCP and DBBM (Bio-Oss®)) with a liquid (cellulose and saline water). The required osteoblasts will be obtained by extracting mesenchymal stem cells from the umbilical cord and inducing their maturation, differentiation and proliferation into osteoblastic cells as well as by obtaining the pulp from extracted teeth and inducing the same process. The created osteoblasts will be cultured and tested with a regular xenograft (DBBM) (control group), then the putty will be tested alone with these osteoblasts (test group 1) and then with the addition of PRF (test group 2). Quality control tests and proliferation tests will be performed. Results will be recorded for statistical analysis. The regenerative properties of the new bone grafting putty will be compared to those of DBBM by grafting the two products in critical size rats’ calvarial defects. The rats will be divided into two groups. For both groups, 2 critical size, circular calvarial defects will be made on either side of the median sagittal suture. The animals will be sacrificed at 4 and 8 weeks and the calvaria will be removed with a surgical bur. Coronal sections of the specimens will then be obtained, stained and analyzed using a light microscope linked to a digital camera and an automated image analysis software. Results will be recorded for statistical analysis.